Coding

Part:BBa_K1357002:Design

Designed by: Matthew Tucker   Group: iGEM14_Virginia   (2014-10-06)


NhaR Transcriptional Regulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 175


Design Notes

This transcriptional activator is easier to express then the entire PGA operon (pgaABCD is 6.5 kb which is much greater than the 0.9kb of NhaR)

Source

PCR Amplified from E.coli K-12 XL1 Blue Cells Genome

References